Species: Serratia marcescens
Product Description: TurboNuclease is an ultra-pure recombinant form of Serratia marcescens extracellular endonuclease (encoded by the same gene as Benzonase) produced in E. coli using a proprietary process. This non-specific endonuclease hydrolyzes both single- and double-stranded nucleic acids (DNA and RNA) to 5’-phosphorylated oligonucleotides of 1-4 bases in length. TurboNuclease is a highly purified homodimer of 27 kDa subunits that has exceptionally high specific activity and is free of protease activity. TurboNuclease is ideal to digest nucleic acids and to reduce viscosity during protein purification and sample preparation.
Application Note: One unit of TurboNuclease converts 1.0 OD260 of salmon sperm DNA into acid-soluble nucleotides in 30 minutes at 37oC in a reaction buffer of 50 mM Tris-HCl, pH 8.0 and 1 mM MgCl2. This corresponds to complete digestion of 50 μg of salmon sperm DNA into oligonucleotides. TurboNuclease has similar specific activity as Benzonase. (For detail information, please refer to the protocol)
Form: Liquid
Buffer (with preservative): 50mM Tris-HCl (pH8.0), 50mM NaCl, 5mM MgCl₂, 50% Glycerol, no preservative.
Concentration: 250 Unit/μl (Please refer to the vial label for the specific concentration.)
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ExpressionSystem: E. coli
Conjugation: Unconjugated
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