Formulation: 50 mM Tris-HCl, pH 7.5, 200 mM sodium chloride, and 20% glycerol
Purity: ≥90%
Formula Weight: 0
Shelf life (days): 365
Notes: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped positive-stranded RNA virus and the causative agent of COVID-19, a primarily respiratory illness characterized by fever, cough, and shortness of breath that can lead to life-threatening complications.{53462,53459,53461,55257,55258} The SARS-CoV-2 genome contains approximately 30 kilobases and 14 open reading frames (ORFs) that encode four structural proteins: spike, envelope, membrane, and nucleocapsid, as well as 16 non-structural proteins and 9 accessory factors.{57176} SARS-CoV-2 non-structural protein 1 (nsp1) is a multifunctional protein and virulence factor that inhibits host mRNA translation, including translation of antiviral proteins such as IFN-β and IFN-λ1.{68723,68724,68725} It is composed of an N-terminal domain and a C-terminal domain connected via an unstructured 20-amino acid residue linker.{68726,68725} The SARS-CoV-2 nsp1 C-terminal domain binds to the mRNA entry site of the 40S subunit of the host ribosome, which prevents host mRNA from entering, while the N-terminal binds to the 40S decoding center preventing accurate alignment of tRNA and mRNA.{68724,68726,68725} SARS-CoV-2 nsp1 also induces host mRNA degradation, prevents mRNA export from the nucleus to the cytoplasm, and binds to pre-rRNA in the nucleolus to disrupt rRNA biogenesis.{68727,68725,68728,68723}
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