Formulation: 50 mM Tris-HCl, pH 7.5, with 200 mM sodium chloride and 20% glycerol
Purity: ≥90%
Formula Weight: 0
Shelf life (days): 365
Notes: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped positive-stranded RNA virus and the causative agent of COVID-19, a primarily respiratory illness characterized by fever, cough, and shortness of breath that can lead to life-threatening complications.{53462,53459,53461,55257,55258} The SARS-CoV-2 genome contains approximately 30 kilobases and 14 open reading frames (ORFs) that encode four structural proteins: spike, envelope, membrane, and nucleocapsid, as well as 16 non-structural proteins and 9 accessory factors.{57176} SARS-CoV-2 non-structural protein 10 (nsp10) is encoded within ORF1ab and is a cofactor for guanine-N7 methyltransferase (guanine-N7 MTase) and 2ʹ-O-methyltransferase (2ʹ-O-MTase).{57176,67303,52794} The amino acid sequence of nsp10 displays few mutations and is highly conserved across SARS-CoV-2 variants.{67303} SARS-CoV-2 nsp10 binds to and stabilizes the disordered N-terminal exoribonuclease domain (ExoN domain) of guanine-N7 MTase and is necessary for accurate exonuclease activity.{67303} It forms a complex with 2ʹ-O-MTase to methylate nascent mRNAs at the ribose 2'-O position, creating a Cap-1 structure that facilitates increased translation of viral mRNAs and reduced innate immune recognition by the host cell.{52794}
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