Products from BPS Bioscience require a minimum order value above 400€
Applications: Useful for inhibition of JAMM-family isopeptidases. Especially valuable for protecting K63-linked polyubiquitin chains in cell lysates.
Biological Activity: Metalloprotease inhibitor; chelates iron, zinc and other divalent metals. Effective concentration 1-10 mM.
CAS Number: 5144-89-8
Description: 1,10-phenanthroline (o-phenanthroline) is a chelator with high affinity for divalent metal ions. When complexed with copper, it possesses nuclease activity that has been used to study DNA-protein interactions. While not specific for DUBs, it has been shown to be a potent inhibitor of JAMM-type isopeptidases through chelation of the active site Zn2+ ion. As such, o-phenanthroline is widely used when preparing cell lysates for enrichment of proteins bearing K63-linked polyubiquitin chains which are especially sensitive to DUBs in the JAMM family.
Format: DMSO solution
Purity: ≥99%
Solubility: Soluble in ethanol or methanol (200 mM).Weakly soluble in aqueous solutions.
Storage Stability: Store powder at room temperature. Store ethanol or methanol stock solutions at -20°C. Aqueous solutions should be used within a few days.
Supplied As: 0.5M solution in anhydrous DMSO (100X)
Warnings: Protect from moisture. Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Cooper, E. M., Cutcliffe, C., Kristiansen, T. Z., Pandey, A., Pickart, C. M., and Cohen, R. E. (2009) K63-specific deubiquitination by two JAMM/MPN+ complexes: BRISC-associated Brcc36 and proteasomal Poh1. EMBO J. 28:621-631.
2. Sigman, D.S., et al., Nuclease activity of 1,10-phenanthroline-copper in study of protein DNA interactions. Methods Enzymol., 208:414-433 (1991).
English
Deutsch
