Formulation: A solid
Formal Name: 3,8-diamino-5-ethyl-6-phenyl-phenanthridinium, monobromide
Purity: ≥95%
Formula Markup: C21H20N3 • Br
Formula Weight: 394,3
Shelf life (days): 1460
CAS Number: 1239-45-8
Notes: Ethidium is a phenanthridine fluorescent dye and DNA intercalator.{72317,72318} It displays an emission at 595 nm upon excitation at 525 nm.{72317} Ethidium inhibits the DNA-unwinding and ATPase activities of P. falciparum DNA helicase (IC50s = 1.5 and 2.5 µM, respectively) and is active against P. falciparum in vitro.{47362} It completely inhibits E. coli RNA polymerase when used at a concentration of 100 µmol/ml and is active against Mycoplasma when used at concentrations ranging from 0.5 to 8 µg/ml.{20743,72319} Ethidium (8 mg/kg) increases lifespan in 6C3HED-OG and L5178Y murine lymphoma models.{72320} It has commonly been used to stain DNA in agarose gel electrophoresis and in displacement assays to determine compound binding to DNA.{38313,22204}
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