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Applications: Forskolin is commonly used to raise levels of cAMP in a wide variety of intact cells and tissue preparations.
Biological Activity: Forskolin is shown to activate adenylate cyclase at EC50 of 5-10 µM. Forskolin binds to adenylyl cyclase in membranes from stably transfected Sf9 cells expressing type 1 adenylyl cyclase with an IC50 value of 41 nM and demonstrates an EC50 value of 0.5 µM in an activation assay assessing formation of cAMP from ATP.
CAS Number: 439081-18-2
Description: Forskolin is a cell permeable adenylate cyclase activator which causes an increase in the intracellular concentration of cAMP. This makes it an important hypotensive and vasodilatory agent. Forskolin also affects calcium currents and inhibits PKC (Protein Kinase C)-stimulated MAPK and RAF-1.
Format: White to off-white granular powder.
Purity: ≥95% by HPLC
Solubility: Soluble in DMSO at 160 mg/ml; very poorly soluble in water; maximum solubility in water is estimated to be about 25-50 µM. Buffers, serum, or other additives may increase or decrease the aqueous solubility. Forskolin is also soluble in ethanol (50 mg/ml), but this solvent is not recommended, as it inhibits forskolin activation of adenylate cyclase.
Storage Stability: Store at or below -20°C. Stable for at least 1 year when stored as directed. Anhydrous DMSO stock solutions are stable for at least 3 months.
Warnings: Protect from moisture. Avoid freeze/thaw cycles of solutions.
Biosafety Level: Not applicable (BSL-1)
References: 1. Awad, et al., J.Biol.Chem 258: 2690 (1983).
2. KB Seamon., et al., PNAS 78 (6):3363 - 3367 (1981).
3. Siddhanti, S.R., et al., Endocrinology 136: 4834 - 4841 (1995).
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