Formulation: A solid
Formal Name: 3′,6′-dihydroxy-3-oxo-spiro[isobenzofuran-1(3H),9′-[9H]xanthene]-6-carboxylic acid, 2,5-dioxo-1-pyrrolidinyl ester
Purity: ≥95%
Formula Markup: C25H15NO9
Formula Weight: 473,4
Shelf life (days): 1460
CAS Number: 92557-81-8
Notes: 6-CFSE is cell-permeable fluorogenic probe that is composed of the amine-reactive fluorescent probe 6-carboxyfluorescein (Item No. 21233) and a cleavable succinimidyl ester.{13893} It is cleaved by intracellular esterases to produce 6-carboxyfluorescein, which displays excitation/emission maxima of 492/518 nm, respectively. The racemic mixture of 6-CFSE and 5-CFSE (Item No. 16802) has been used to determine the intracellular pH of bacteria, quantify cell division in vitro and in vivo, and track lymphocyte migration in vivo.{36590,17820,17802}