Product Description: Coelenteramine 400a (Coelenterazine 400a), a derivative of Coelenterazine, is a Renilla luciferase (RLuc) substrate. In the presence of Coelenteramine 400a, RLuc can emit blue light at 395 nm[1][2]. Coelenterazine 400a will causes color change in the bioluminescence reaction of Rluc by replacing the sulfur and oxygen heteroatoms of the methylene bridge. Coelenterazine 400a provides higher signal resolution and can be used in the research of bioluminescence resonance energy transfer (BRET)[3].
Formula: C26H21N3O
References: [1]Levi J, et, al. Bisdeoxycoelenterazine derivatives for improvement of bioluminescence resonance energy transfer assays. J Am Chem Soc. 2007 Oct 3;129(39):11900-1. /[2]Bertrand L, et, al. The BRET2/arrestin assay in stable recombinant cells: a platform to screen for compounds that interact with G protein-coupled receptors (GPCRS). J Recept Signal Transduct Res. Feb-Nov 2002;22(1-4):533-41./[3]Yuan M L, et al. Luminescence of coelenterazine derivatives with C-8 extended electronic conjugation[J]. Chinese Chemical Letters, 2016, 27(4): 550-554.
CAS Number: 70217-82-2
Molecular Weight: 391.46
Compound Purity: 99.70
Research Area: Others
Solubility: DMSO : 8.33 mg/mL (ultrasonic;warming;heat to 60°C)/Ethanol : 1 mg/mL (ultrasonic;warming;heat to 60°C)
Target: Fluorescent Dye
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