Product Description: Ethidium monoazide bromide is a DNA intercalating fluorescent dye that enters bacteria with damaged membranes. Ethidium monoazide bromide can be covalently linked to DNA by photoactivation. Ethidium monoazide bromide stains only dead cells[1]. Ethidium monoazide (bromide) is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. Strain-promoted alkyne-azide cycloaddition (SPAAC) can also occur with molecules containing DBCO or BCN groups.
Formula: C21H18BrN5
References: [1]Nogva HK, et al. Ethidium monoazide for DNA-based differentiation of viable and dead bacteria by 5'-nuclease PCR. Biotechniques. 2003 Apr;34(4):804-8, 810, 812-3.
CAS Number: 58880-05-0
Molecular Weight: 420.31
Research Area: Others
Solubility: 10 mM in DMSO
Target: DNA Stain
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