Product Description: Flubi-2 is a fluorescein-biotin based pH-ratio dye (Ex=480 nm, Em= 520-560 nm) with a pK value of 6.7. Flubi-2 is a hydrolysis product of Flubida-2 (non-fluorescent, membrane permeable). Flubi-2 can be used for pH determination of organelles of the secretory pathway (such as golgi apparatus and endoplasmic reticulum) in living cells[1][2].
Formula: C39H44N4O8S
References: [1]Wu MM, et al. Studying organelle physiology with fusion protein-targeted avidin and fluorescent biotin conjugates. Methods Enzymol. 2000;327:546-64. /[2]Wu MM, et al. Organelle pH studies using targeted avidin and fluorescein-biotin. Chem Biol. 2000 Mar;7(3):197-209.
Molecular Weight: 728.85
Research Area: Others
Solubility: 10 mM in DMSO
Target: Fluorescent Dye