Product Description: Propidium monoazide is a photoreactive DNA-binding dye that preferentially binds to dsDNA. Propidium monoazide (PMA) prevents DNA from dead bacteria from being amplified during the PCR. PMA-PCR enhanced both the specificity and the sensitivity of PCR[1]. Propidium monoazide is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. Strain-promoted alkyne-azide cycloaddition (SPAAC) can also occur with molecules containing DBCO or BCN groups.
Formula: C27H32Cl2N6
References: [1]Mohamed Askar 1, et al. Propidium monoazide-polymerase chain reaction for detection of residual periprosthetic joint infection in two-stage revision. Mol Biol Rep. 2019 Dec;46(6):6463-6470.