Caspase 1 Activity Assay Kit(Colorimetric Method)

Assay Time: 4-6 hours

Test Principle: Caspase 1, also known as Interkeukin 1β Conberting Enzyme (ICE), is the only caspase in the caspase family that can cleave IL-1β prefixes or IL-18 prefabrication. Caspase 11 can cleave 45 kD caspase 1 preparation to produce 20 kD and 10 kD fragments, which can form heterodimer and further form tetramer by two heterodimers. Caspase 1 regulates apoptosis by cleaving Bcl-XL and the associated immune response by cleaving cytokine precursors, or regulates pyroptosis by hydrolyzing Gastermin D (GSDMD). This caspase 1 activity assay kit is based on caspase 1 that can catalyze the substrate AC-YVAD-pNA to generate yellow pNA (p-Nitroaniline). The pNA has a strong absorption near 405 nm, The activity of caspase 1 can be calculated by determining the absorbance at 405 nm.

• SKU: ELSE-CK-A381-20
• Manufacturer: Elabscience Biotechnology
• Manufacturer SKU: ELA-E-CK-A381-20
• Package Unit: 20 Assays
• Quantity Unit: PAK