Formulation: A solution in ethanol
Formal Name: (3β)-[6-[[(2S)-2-amino-(5α)-[(aminoiminomethyl)amino]-1-oxopentyl]amino]hexanoate], cholestan-3-ol, bis(2,2,2-trifluoroacetate)
Purity: ≥95%
Formula Markup: C39H73N5O3 • 2CF3COO
Formula Weight: 886,1
Shelf life (days): 365
CAS Number: 1609010-59-4
Notes: 2H-Cho-Arg is a steroid-based cationic lipid that contains a 2H-cholesterol skeleton coupled to an L-arginine head group and can be used to facilitate gene transfection.{44005} It forms a complex with plasmid DNA (pDNA) and decreases pDNA migration in an electrophoretic mobility shift assay at +/- charge ratios of 4 or higher. 2H-Cho-Arg facilitates transfection of a luciferase gene into H1299 cells, an effect that is reversed by the lipid raft-mediated endocytosis inhibitor methyl-β-cyclodextrin (Item No. 21633) and the caveolae-mediated endocytosis inhibitor genistein (Item No. 10005167), but not by inhibitors of clathrin- or micropinocytosis-mediated endocytosis. It induces cytotoxicity in H1299 cells (IC50 = 92.7 μg/ml).
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