Formulation: 1 mM Sodium citrate, pH 6.4
Shelf life (days): 180
Notes: EZ Cap™ Cy5 Firefly Luciferase mRNA encodes for luciferase, a protein originally isolated from the firefly P. pyralis, that catalyzes the ATP-dependent oxidation of the molecule luciferin resulting in chemiluminescence at a wavelength of approximately 560 nm.{31946} It is capped using a co-transcriptional capping method, resulting in the naturally occurring Cap 1 structure with high capping efficiency. EZ Cap™ Firefly Luciferase mRNA is also polyadenylated and modified with 5-methyoxy-UTP (mo5U) to reduce host cell immune response, increase mRNA stability, and enhance translation initiation. Cy5 (Item No. 30752) is a cyanine-containing fluorochrome with excitation/emission maxima of 650/670 nm, respectively.{42160} Encapsulation of EZ Cap™ Cy5 Firefly Luciferase mRNA (mo5U) in lipid nanoparticles (LNPs) can be used for mRNA delivery and expression of luciferase protein in vitro.{69885}
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