Clone: 3G8.
Conjugate: FITC.
Immunogen: Human polymorphonuclear leukocytes.
Storage Buffer: The reagent is provided in aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium azide (NaN3).
Recommended Usage: CD16 clone 3G8, mAb is used for identify/enumerate human antigen (FcRIII). It´s an antipan FcγRIII (CD16) whereas clone GRM1 recognizes NA2-FcRIIIb & FcRIIIa. It´s effective for direct IF staining of human tissue for FCM tests using ≤1 μg/106 cells.
Description: This antibody reacts with the CD16-antigen. CD16 exists as two distinct isoforms, FcγRIIIA and FcγRIIIB. In humans, FcγRIIIA is expressed as a polypeptide-anchored form on monocytes, macrophages, and lymphocytes such as NK cells. T and B cells do not express this Fc receptor. FcγRIIIB is also detected on neutrophils as a GPI-anchored form. Expression of CD16 on lymphocytes and monocytes is similar in non-human primates. Binding of IgG leads to activation of signal transduction pathways, resulting in antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, cytokine release, and antigen presentation.
This monoclonal antibody has been reported to have several functional activities, including inhibition of cytotoxic ability, activation of cell signaling, and NK cell depletion in vivo. Moreover, the 3G8 antibody clone has been demonstrated to work on capuchin monkey, chimpanzee, common marmoset, cynomologous monkey, hamadyras baboon, olive baboon, pigtailed macaque, rhesus, and squirrel monkey.
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