Clone: 1043
Background: The extent of expression of the basement membrane component type IV collagen may be correlated with the degree of differentiation of basement membrane producing neoplasma. Neoplastic cells of invasive carcinomas often lack a continuous basement membrane. In diagnostic histopathology antibodies to type IV collagen may help to distinguish between non-invasive (benign or in situ) and invasive lesions. Furthermore the antiserum is important in the differential diagnosis of bullous lesions in dermatopathology.
Source: 1043 is a mouse monoclonal IgG1 antibody derived by fusion of Sp 2/0 mouse myeloma cells with spleen cells from a BABL/c mouse immunized with purified human type IV collagen. The immunogen has been isolated from human placenta.
Specificity: 1043 recognizes type IV collagen in the extracellular - basement membranes of normal and pathological human tissues.
Formulation: Each vial contains 100 ul 1mg/ml of purified monoclonal antibody in PBS containing 0.09% sodium azide
References: 1. Visser, R., van der Beek, J.M.H., Havenith, M.G., Cleutjens, J.P.M. and Bosman, F.T. (1986). Immunocytochemical detection of basement membrane antigens in the histopathological evaluation of laryngeal dysplasia and neoplasia. Histopathology 10, 171-180.2. Cleutjens, J.P.M., Havenith, M.G., Kasper, M., Vallinga, M., and Bosman, F.T. (1990). Absence of type IV collagen in the centre of the corneal epithelial basement membrane. Histochemical Journal 22, 688-94. 3. Havenith, M.G. et al. (1987). Human specific anti-type IV collagen monoclonal antibodies, characterisation and immunohistochemical appliCation. Histochemistry 87, 123-28. 4. Schapers, R.F.M., Pauwels, R.P.E., Havenith, M.G., Smeets, A.W.G.B, van den Brandt and P.A. Bosman, F.T. (1990). Prognostic Significance of type IV collagen and laminin immunoreactivity in urothelial carcinomas of the bladder. Cancer 66, 2583-88.5. Cleutjens JP, Havenith MG, Beek C, Vallinga M, Ten Kate J, Bosman FT. (1990) Origin of basement membrane type IV collagen in xenografted human epithelial tumor cell lines. Am J Pathol. 136:1165-72.
UniProt: P08572, Q01955, P29400, P53420, P02462
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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