Clone: Bp53.12
Background: AlteRations of the p53 tumor suppressor gene are considered critical events in multistage carcinogenesis of a wide range of Human cancers. The mutations found in sporadic cancer are commonly mis-sense mutations that inactivate the tumor suppressor activity of p53. These mutant proteins are much more stable than the normal p53 protein, frequently adopt an altered conformation and accumulate within the malignant cells.
Source: Bp53.12 is a Mouse monoclonal IgG2a antibody derived by fusion of X63 Ag8.653 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with recombinant Human wild-type p53 protein.
Specificity: Bp53.12 reacts with a denatuRation-resistant epitope of p53. This antibody is specific for Human p53 and recognizes amino acid sequence 16-25.
Formulation: Each vial contains 100 ug 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
References: 1. Bartek, J., Bartkova, J., Vojtesek, B., Staskova, Z., Lukas, J., Rejthar, A., Kovarik, J., Midgley, C. A., Gannon, J. V., and Lane, D. P. (1991). Aberrant expression of the p53 oncoprotein is a common feature of a wide spectrum of Human malignancies. Oncogene 6, 1699-1703. 2. Bartek, J., Bartkova, J., Lukas, J., Staskova, Z., Vojtesek, B., and Lane, D. P. (1993). Immunohistochemical analysis of the p53 oncoprotein on paraffin sections using a series of novel monoclonal antibodies. J Pathol 169, 27-34.
UniProt: P04637
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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