Clone: 123A8
Background: NCAM / CD56, as a member of the immunoglobulin superfamily of adhesion molecules is characterized by several immunoglobulin (Ig)-like domains. The extracellular part of NCAM consists of five of these Ig domains and two fibronectin type III homology regions. NCAM is encoded by a single copy gene composed of 26 exons. However, at least 20-30 distinct isoforms can be generated by alternative splicing and by post-translational modifications, such as sialylation. During sialylation, polysialic acid (PSA) carbohydrates are attached to the extracellular part of NCAM. Through its extracellular region, NCAM mediates homophilic interactions. In addition, NCAM can also undergo heterophilic interactions by binding extracellular matrix components, such as laminin, or other cell adhesion molecules, such as integrins.NCAM can be found in central and peripheral nerve cells, neuroendocrine tissues and at the surface of NK-cells. Also, NCAM is present in malignancies derived from these tissues and cells.
Source: 123A8 is a mouse monoclonal IgG1 antibody derived by fusion of mouse myeloma cells with cells from a mouse immunized with a membrane preparation of a human small cell lung carcinoma
Specificity: The antibody 123A8 is directed against human NCAM/CD56 and reacts with an epitope within the membrane-proximal region of the NCAM molecule. The antibody binds to all NCAM isoforms.
Formulation: Each vial contains 100µg 1mg/ml purified monoclonal antibody in phosphate buffered saline (PBS) containing 0.09% sodium azide.
References: 1.Moolenaar CE, Muller EJ, Schol DJ, Figdor CG, Bock E, Bitter-Suermann D, Michalides RJ.(1990). Expression of neural cell adhesion molecule-related sialoglycoprotein in small cell lung cancer and neuroblastoma cell lines H69 and CHP-212. Cancer Res. 50:1102-1106.2.Langdon SP, Hawkes MM, Hay FG, Lawrie SS, Schol DJ, Hilgers J, Leonard RC, Smyth JF. (1988). Effect of sodium butyrate and other differentiation inducers on poorly differentiated human ovarian adenocarcinoma cell lines. Cancer Res. 48:6161- 6165.3.Langdon SP, Lawrie SS, Hay FG, Hawkes MM, McDonald A, Hayward IP, Schol DJ, Hilgers J, Leonard RC, Smyth JF. (1988). Characterization and properties of nine human ovarian adenocarcinoma cell lines. Cancer Res. 48:6166-6172.4.Gerardy-Schahn R, Eckhardt M. (1994). Hot spots of antigenicity in the neural cell adhesion molecule NCAM. Int J Cancer Suppl.8:38-42.5.Kwa HB, Wesseling J, Verhoeven AH, van Zandwijk N, Hilkens J. (1996). Immunoscintigraphy of small-cell lung cancer xenografts with anti neural cell adhesion molecule monoclonal antibody, 123C3: improvement of tumour uptake by internalisation. Br J Cancer. 73:439-446.6.Advances in the applications of monoclonal antibodies in clinical oncology. (1987). Abstracts. Br J Cancer. 56:509-530.
UniProt: O15394
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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