Clone: UZ4
Background: Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes. In addition, leukocyte adhesion occurs in the capillaries and small venules at any site in the body after onset of inflammation. This response is immediate and involves a cascade of adhesion receptors. At the endothelial surface members of the selectin and immunoglobulin superfamilies participate in this cascade. Selectins are C-type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. E-selectin (CD62E) is an endothelial cell specific selectin that is expressed only after activation with proinflammatory cytokines. In vitro experiments have shown that IL-1, TNFα and bacterial wall components like lipopolysaccharides induce the transcription of E-selectin in a NFκB dependent signalling cascade. E-selectin has been associated with blood vessel endothelium in diverse inflammatory situations.
Source: UZ4 is a Rat monoclonal IgM antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a Lewis Rat immunized with LPS-activated mlEND1 cells expressing E-selectin at their cell surface.
Specificity: UZ4 recognizes the minimal amino acid consensus sequence CXKKKL present in the lectin domain of both murine and Human E-selectin.
Formulation: Each vial contains 100ul 1 mg/ml purified monoclonal antibody in PBS containing 0,09% sodium azide.
References: 1. Hallmann, R., Zimmermann, U., Sorokin, L. M., Needham, L., and Von der Mark, K. (1995). Adhesion of leukocytes to the inflammed endothelium, Scand J Rheumatol Suppl 101, 107-9. 2. Austrup, F., Vestweber, D., Borges, E., Lohning, M., Brauer, R., Herz, U., Renz, H., Hallmann, R., Scheffold, A., Radbruch, A., and Hamann, A. (1997). P- and E-selectin mediate recruitment of T-helper-1 but not T-helper-2 cells into inflammed tissues, Nature 385, 81-3. 3. Borges, E., Tietz, W., Steegmaier, M., Moll, T., Hallmann, R., Hamann, A., and Vestweber, D. (1997). P-selectin glycoprotein ligand-1 (PSGL-1) on T helper 1 but not on T helper 2 cells binds to P-selectin and supports migRation into inflamed skin, J Exp Med 185, 573-8. 4. Tietz, W., Allemand, Y., Borges, E., von Laer, D., Hallmann, R., Vestweber, D., and Hamann, A. (1998). CD4+ T cells migRate into inflamed skin only if they express ligands for E- and P-selectin, J Immunol 161, 963-70. 5. Alferink, J., Tafuri, A., Vestweber, D., Hallmann, R., Hammerling, G. J., and Arnold, B. (1998). Control of neonatal tolerance to tissue antigens by peripheral T cell trafficking, Science 282, 1338-41. 6. Wolf, D., Hallmann, R., Sass, G., Sixt, M., Kusters, S., Fregien, B., Trautwein, C., and Tiegs, G. (2001). TNF-alpha-induced expression of adhesion molecules in the liver is under the control of TNFR1--relevance for concanavalin A-induced hepatitis, J Immunol 166, 1300-7. 7. Hammel, M., Weitz-Schmidt, G., Krause, A., Moll, T., Vestweber, D., Zerwes, H. G., and Hallmann, R. (2001). Species-specific and conserved epitopes on Mouse and Human E-selectin important for leukocyte adhesion, Exp Cell Res 269, 266-74. 8. Sunderkotter, C., Seeliger, S., Schonlau, F., Roth, J., Hallmann, R., Luger, T. A., Sorg, C., and Kolde, G. (2001). Different pathways leading to cutaneous leukocytoclastic vasculitis in mice, Exp Dermatol 10, 391-404. 9. Kneilling, M., Reinhard, M., Hültner, L., Schönberger, T., Fuchs, K., Schaller, M., Bukala, D., Massberg, S., Sander, C.A., Braumüller, H., Eichner, M., Maier, K.L., Hallmann, R., Pichler, B.J., Haubner, R., Gawaz, M., Pfeffer, K., Biedermann, T., Röcken, M. (2009). Direct crosstalk between mast cell-TNF and TNFR1-expressing endothelia mediates local tissue inflammation. Blood, 114, 1696-1706.
UniProt: P16581
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
English
Deutsch
