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Human A-431 cells were cultured in DMEM medium and harvested at the log phase of growth. The A-431 cells were fixed in 4% paraformaldehyde and arrayed on a 12-well (5 mm) adhesive coated slide, with each wellis surface specifically treated to enhance cellular attachment and to minimize background staining. Each well was loaded with approximately 7.5 x 103 A-431 cells. A follow-up heat dehydration process was done to ensure the attachment of A-431 cells and the stability of cellular proteins.
Application Note: A-431 cell slide is provided for immunocytochemical analysis of protein and protein-protein interaction. Procedures for immunohistochemistry can be directly applied.
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