BIOTIN BETA GALACTOSIDASE Conjugated

Conjugation: Biotin

Description of Target: Biotin is a small biomolecule important for many cellular processes. Most importantly for biotechnology applications, biotin is ammenable to conjugation to proteins for use in biochemical assays. Biotin has a very strong affinity for avidin and streptavidin; an attraction that is the strongest and most stable non-covalent interaction known. Beta-galactosidase (beta-gal; beta-gal) is an enzyme that hydrolyzes beta-galactosides (such as lactose and various glycoproteins) into simple sugars. Beta-galactosidase is routinely used as a reporter in biochemical experiments as well as a marker for gene expression experiments in E. coli. Beta-galactosidase can be used to cleave the chemical X-gal that forms an intense blue product that can easily be quantified.

Molecular Weight: 455

Product Format: Liquid 0.05M Potassium Phosphate, 0.001M Magnesium Chloride, 0.1M Beta-Mercaptoethanol (pH 7.8)

Purification: Biotin Beta Galactosidase Conjugated was prepared from electrophoretically pure Beta Galactosidase and Biotin.

Specificity#: Biotin Beta Galactosidase assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Beta Galactosidase (E.coli) and anti-Biotin.

• SKU: AVIOORA00149
• Manufacturer: Aviva Systems Biology
• Manufacturer SKU: OORA00149-1MG
• Green Labware: No
• Package Unit: 1 mg
• Quantity Unit: STK
• Reactivity: Biotin
• Application: Western Blotting, Immunohistochemistry, Dot Blot, Enzyme-linked Immunosorbent Assay