Product Description: Streptavidin is a 55 kDa (subunit MW 14 kDa) biotin-binding protein isolated from Streptomyces avidini. Streptavidin is superior to avidin, because it does not contain carbohydrate like avidin and has no net charge at neutral pH. Streptavidin~biotin system is routinely used in Immunohistochemistry (IHC). Extinction Coefficient 1% A280=32.0; Eletrophoretically homogenous, single band; Protein content: > 95%;
Application Note: WB: 1:1,000- 1:10,000 (0.1-1.0 µg/ml). ELISA: 1:2,000-1:20,000 (50-500 ng/ml). IHC: 1:200-1:1,000 (1-5 µg/ml). *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Form: Liquid
Buffer (with preservative): 10mM Tris-HCl, 200mM NaCl (pH8.0), 50% Glycerol buffer, 10 mg of BSA, 0.05% Sodium azide.
Concentration:
Background: Streptavidin is a 55 kDa (subunit MW 14 kDa) biotin-binding protein isolated from Streptomyces avidini. Streptavidin is superior to avidin, because it does not contain carbohydrate like avidin and has no net charge at neutral pH. Streptavidin~biotin system is routinely used in Immunohistochemistry (IHC). Extinction Coefficient 1% A280=32.0; Purity: > 99% pure by HPLC; Eletrophoretically homogenous, single band;Protein content: > 95%; Activity 14.8 units/ mg, one unit bunds 1.0 microgram of d-biotin; Protease activity: 0.000018 units/mg; Isoelectric point: 7.0 +/- determined by Isoelectric focusing. Alkaline Phosphatase (ALP) used in this conjugation is 140 KDa enzyme purified from calf intestine.
Conjugation: AP
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