Purification: Pure Streptavidin is reacted with Succinimidyl 4- (N-maleimido-methyl) cyclohexane - 1- carboxylate (SMCC). Allophycocyanin is reacted with N-succinimidyl 3-(2 - pyridyldithio) propionate (SPDP) and activated by reduction with dithiothreitol. The two substituted proteins are reacted together to give covalent conjugates, which are selected by medium pressure liquid chromatography on gel filtration columns of AcA34 ultrogel.
Specificity#: This product is designed as a secondary reagent for use in flow cytometry in conjunction with biotinylated primary antibodies. This product conjugates show negligible non-specific binding to non-biotinylated macromolecules, and therefore gives very low backgrounds.