Product Description: Streptavidin is a tetrameric protein capable of binding 4 biotin groups to each molecule of streptavidin. While streptavidin has identical binding properties as avidin, it lacks the glycoprotein portion of the molecule and therefore shows less non-specific binding. Streptavidin is a slightly smaller molecule with a molecular weight of approximately 53.6 kDa. The sequence of avidin only shows 30% homology with streptavidin, and anti-avidin and anti-streptavidin antibodies are not immunologically cross reactive. Horseradish Peroxidase (HRP) is an enzyme that utilize organic peroxide compounds as electron donors.
Application Note: WB: 1:10,000-1:40,000. IHC-P: 1:1,000-1:5,000. ELISA: 1:20,000-1:200,000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Form: Liquid
Buffer (with preservative): 0.02M Potassium Phosphate (pH7.2), 0.15M Sodium Chloride, no preservative.
Concentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
ExpressionSystem: Streptomyces avidinii
Conjugation: HRP
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