Conjugation: R-PE
Description of Target: STREPTAVIDIN:RPE
Key Reference: 1. Van der Nieuwenhof, I. et al. (2001) Differential galactosylation of neuronal and haematopoietic signal regulatory protein-alpha determines its cellular binding - specificity. J. Cell. Sci. 114 (7): 1321 - 1329
2. Tasker, L. et al. (1997) Immature B cells from neonatal mice show a selective inability to up-regulate MHC class II expression in response to antigen receptor ligation. Int. Immunol. 9: 475 - 484.
Product Format: Lyophilized. Phosphate buffered saline
Protein Size: 159
Purification: Pure Streptavidin is reacted with Succinimidyl 4- (N-maleimido-methyl) cyclohexane - 1- carboxylate (SMCC). R. Phycoerythrin is reacted with N-succinimidyl 3-(2 - pyridyldithio) propionate (SPDP) and activated by reduction with dithiothreitol. The two substituted proteins are reacted together to give covalent conjugates, which are selected by medium pressure liquid chromatography on gel filtration columns of AcA34 ultrogel. STAR4A conjugates show negligible non-specific binding to non-biotinylated macromolecules, and therefore gives very low backgrounds.
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