Product Description: Streptavidin is a 55 kDa (subunit MW 14 kDa) biotin-binding protein isolated from Streptomyces avidini. Streptavidin is superior to avidin, because it does not contain carbohydrate like avidin and has no net charge at neutral pH. Streptavidin~biotin system is routinely used in Immunohistochemistry (IHC). Extinction Coefficient 1% A280=32.0; Protein content: > 95%; Texas Red: A max. 596 nm; E max. 620 nm. Fluorochrome / protein Absorbance ratio: A596 nm/ A280 nm.
Form: Liquid
Buffer (with preservative): 10mM Phosphate, 150mM NaCl, 1% BSA, 0.05% Sodium azide.
Concentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
Background: Streptavidin is a 55 kDa (subunit MW 14 kDa) biotin-binding protein isolated from Streptomyces avidini. Streptavidin is superior to avidin, because it does not contain carbohydrate like avidin and has no net charge at neutral pH. Streptavidin~biotin system is routinely used in Immunohistochemistry (IHC). Extinction Coefficient 1% A280=32.0; Purity: Streptavidin used is 99% pure by HPLC; Eletrophoretically homogenous, single band, Protein content: > 95%; Activity 14.8 units/ mg, one unit bunds 1.0 microgram of d-biotin; Protease activity: 0.000018 units/mg; Isoelectric point: 7.0 +/- determined by Isoelectric focusing. Texas Red: A max. 596 nm; E max. 620 nm. Fluorochrome / protein Absorbance ratio: A596 nm/ A280 nm.
Conjugation: TxRd
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