Application Note: This immunohistochemistry protein blocker is specifically designed for immunohistochemical staining where detection occurs using an immunoenzymatic reaction with substrate, i.e. peroxidase (HRP) with diaminobenzidine (DAB). This product specifically inhibits non-specific staining of IHC detection reagents. It may be used on various specimens, including cultured cells and primary tissue to lower background staining. Optimum performance is achieved using this product undiluted. However, Immunohistochemistry Protein Blocking Buffer can often be diluted 1:1 in TBS without a significant loss of performance. This immunohistochemistry protein blocker consists of proprietary blocking agents and stabilizers prepared using ultra pure reagents dissolved in pharmaceutical grade water (WFI). The buffer is pH adjusted to pH 7.6.
Concentration Value: 1X
Immunohistochemistry Dilution: Undiluted or 1:1
General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Physical State: Liquid (sterile filtered)
Purity and Specificity: Blocking Buffer for Immunohistochemistry (Serum And Azide Free) was aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.
Background: A detailed protocol for immunohistochemical staining is available at www.rockland.com.
Low Endotoxin: No
English
Deutsch
