Application Note: Bovine Serum Albumin - Fraction V is suitable for use in lateral flow, in protease sensitive assays such as RIA, EIA and nucleic acid hybridization, use as a stabilizing agent for proteins and enzymes including dilute solutions of antibody, use as a blocking agent to reduce non-specific binding for FACS, IF, IHC, and WB.
ELISA Dilution: User Optimized
FLISA Dilution: User Optimized
Flow Cytometry Dilution: User Optimized
Immunohistochemistry Dilution: User Optimized
IF Microscopy Dilution: User Optimized
Immunoprecipitation Dilution: User Optimized
Western Blot Dilution: User Optimized
General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Other Performance Data: Purity (%): 100% by Agarose Zone electrophoresisMoisture (%) 0.9 % Loss on DryingpH: 7.0 Protein (%): 100.5 % by nitrogen analysis. Protease: <0.005 Units/mg Heavy Metals: < 0.49 ppm by ICPEndotoxin: < 0.1 EU/mg by LAL
Physical State: Lyophilized
Purity and Specificity: Purity (%): 100% by Agarose Zone electrophoresisMoisture (%) 5% Loss on DryingpH: 7.0Ash: <2.0%Protein (%): 98% by nitrogen analysisProtease: <0.005 Units/mgHeavy Metals: (Pb) < 10 ppm by ICPEndotoxin: < 3 EU/mg by LALIgG: Not DetectedNEFA: N/A
Background: Bovine Serum Albumin (BSA) is used for various biochemical applications including ELISA (Enzyme-Linked Immunosorbent Assay), high content screening assays, western blotting, FACS Buffer and immunohistochemistry. BSA as a blocking reagent is particularly useful with casein-sensitive antibodies, such as phospho-specific antibodies. Also used as a nutrient in cell and microbial culture. In restriction digests, BSA is used to stabilize some enzymes during digestion of DNA and to prevent adhesion of the enzyme to reaction tubes and other vessels. Bovine Serum Albumin can also be used to determine the quantity of other proteins, by comparing an unknown quantity of protein to known amounts of BSA.
Low Endotoxin: No
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