Product Description: Trypsin Antigen Retrieval Kit (2 components)
Application Note: To one ml of buffer (reagent B), add 2-4 drops of concentrated enzyme (reagent E) in a test tube, mix well. This ready-to-use reagent is good for 2-3 hours.1. Deparafinize FFPE tissue section is carried out as outlined in your protocol. Hydrate tissue with PBS.2. Remove buffer, add 2-4 drops of ready-to use enzyme solution to cover the tissue section.3. Incubate at 37ºC for 5-10 minutes or 10-15 minutes at room temperature, (the time required for optimum digestion of FFPE tissue section will vary with the extent of fixation, generally approx. 5-15 minutes are sufficient at the above temperatures. In some cases it may require more time).4. Discard trypsin reagent, wash 3-5 times with buffer.5. The tissue sections are ready for further IHC protocol.
Form: Liquid
Concentration:
Background: Trypsin is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin; mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. Trypsin digestion improves immunoreactivity of some antigens in FFPE tissue sections. For cytokeratin clone AE3 and AE1/AE3, this enzyme works much better than boiling the tissue with citrate buffer.
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