Product Description: Hematoxylin - immuno/histo aqueous
Application Note: Please refer to protocol for details.
Concentration:
Background: This product is water- based, unlike the most common hematoxylins available which are alcohol-based. The use of alcohol-based hematoxylin destroys most of the chromogens (i.e., AEC, Fast Red, BCIP/INT, etc.) used for immunocyto-chemistry. This hematoxylin formulation avoids this problem and is specifically designed for nuclear counterstaining using automated and/or manual immunocytochemistry techniques. This hematoxylin provides a clean, strong and permanent nuclear stain in the formalin-fixed, paraffin-embedded tissue sections, as well as in frozen tissue sections.The bright blue color is stable for 2-3 years if kept in the dark. The counterstain can be used with HRP chromogens, AEC, DAB and AP chromogens, fast red, BCIP/INT and super fast red. This hematosylin is also suitable for H and E stainingt in histology. Slides stained with this hematoxylin can be mounted with aqueous or organic mounting mediums.
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