Product Description: Alkaline Phosphatase Enhancer
Application Note: 1. After the alkaline phosphatase reaction, wash slide with buffer, and distilled/ deionized water.
2. Add few drops of this ready to use Enhancing Reagent to cover the tissue/cell smear, for 1-2 minutes and discard. DO NOT WASH SLIDE.
3. Add few drops of ready to use alkaline phosphatase chromogen, follow instructions as outlined for these chromogens.
Concentration:
Background: Alkaline Phosphatase (alk. phos.), an enzyme derived from bovine intestine mucosa is used as a label in situ hybridization and Immunohistochemistry (IHC).Several insoluble chromogens are used to detect this enzyme. This stable solution is used to increase the signal generated by alkaline phosphatase. It can be used with Fast red, BCIP/INT, BCIP/NBT, our Fast Red Super or any other alk phos. chromogen. A single treatment of this reagent is applied before the chromogen.
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