Application Note: BCIP/TNBT Membrane Alkaline Phosphatase Substrate is a useful detection reagent for Western blot, Immunohistochemistry, and in situ hybridization.
Immunohistochemistry Dilution: 1X
Western Blot Dilution: 1X
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Physical State: Liquid - clear to very faint yellow/orange
Purity and Specificity: pH: 9.28 +/- 0.1QC Raw Material: PASSAbsorbance check of final product: PASSPerformance Data per Immunoblot: PASS
Background: BCIP/TNBT (5-Bromo-4-chloro-3'-indolyphosphate p-toluidine salt/ Tetrazolium nitro blue tetrazolium chloride) is utilized in combination as a chromogenic substrate for alkaline phosphatase (AP) in various applications, including WB and IHC. The reaction produces an insoluble dark purple/blue precipitate. This substrate is particularly useful for chromogenic detection in blotting and in situ hybridization assays due to its high sensitivity and resolution.
Low Endotoxin: No
Other: User Optimized
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