Product Description: DAB Chromogen/HRP Substrate kit - Liquid Format
Application Note: Components:
1) 5mL concentrated amber-colored DAB Chromogen Solution.
2) 200mL clear Stable DAB/Plus Substrate Buffer.
3) One empty mixing dropper bottle.
Working Solution:
Aliquot 1mL of Stable DAB/Plus Buffer in mixing bottle. Add 20μL (one drop) ofconcentrated Stable DAB/Plus Chromogen. Replace tip and mix.
Testing Procedure:
1.) After peroxidase incubation, wash tissue sections with wash buffer.
2.) Wipe slides removing excess buffer. Add enough drops of working Stable DAB/Plussolution to cover tissue sections.
3.) Incubate for 5-10 minutes at room temperature.
For optimal results, observe reaction under the microscope for signal development.Once the desired signal to noise ratio is achieved, stop the reaction by washing slides in buffer.
Note: The working Stable DAB/Plus solution is stable for at least 2 weeks and should beprepared in an opaque bottle. Store at 2-8ºC when not in use. Any solution not used afterthis period should be discarded.
Form: Liquid
Concentration:
Background: Peroxidase from the antibody detection system reacts with H2O2 substrate to degrade it,which then reacts with DAB, precipitating it at positive sites yielding a dark brown color.
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