Intended use: For Research Use Only. The mounting medium (water based), is intended for coverslipping from aqueous solutions.
Summary and explanation: The aqua mounting medium is a widely used for coverslipping from aqueous solutions and is non-fluorescing and has an antifade component to increase the viewing time of the specimen. Use Aqua-Poly/Mount with most fluorescent dyes and stains including DAB, Alkaline Phosphatase, Fast Red, AEC (aminoethylcarbazole) and a variety of other chromogens to enhance and retain fluorescent intensity. The mounting medium (water based) can be used for frozen sections, fat stains, chromogens for immunohistochemistry and in situ hybridization as well as other applications requiring a water-soluble mounting medium.
Reagents provided: 50 ml Mounting medium (water based), ready-to-use.
Storage and handling: Store at room temperature
Principle of method: Coverslipping from aqueous solutions
Procedure: 1. Prepare slides as required.2. Prior to coverslipping rinse the slides in distilled or deionized water.3. Place the bottle upside down with the end cap attached in a container before use. This will help clear of tinybubbles.4. Blot excess water from the slide without drying the tissue specimen. The tissue must be moist prior to mounting.5. Apply enough drops of aqua mounting medium on the tissue section so that the tissue is completely covered.6. Carefully lower the coverslip at an angle while gently applying pressure to force any excess medium and airbubbles away from the tissue and out from under the coverslip.7. Slides can be dried at room temperature or at 4°C or in an oven but the temperature hight is depending whattype of staining is used.8. Allow the aqua mounting medium to dry before examine under the microscope.
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