Product Description: Fluoroshield™ with Phenylenediamine anti-fading medium
Application Note: Apply 3-4 drops of mounting medium directly on top of the specimen and spread out evenly by tilting back and forth or spread evenly with a 0.2 ml plastic pipette tip making sure the tissue is not touched. Excess medium can be removed by touching the edges of the slide on a paper towel. Let stand at room temperature for about 5 minutes. Apply cover slip, carefully avoiding air bubbles.
Form: Liquid
Concentration:
Background: Fluoroshield with strong antifading agent, 1,4-phenylenediamine (PPD) is an aqueous mounting medium for preserving fluorescence of tissue and cell smears. This unique formula prevents rapid photobleaching of FITC, Texas Red, AMCA, Alexa fluoro 488, Alexa fluoro 594, tetramethyly rhodamine, and Redox. The fluorescence is retained during prolonged storage at 4oC in the dark. This medium contains phenylenediamine and is not suitable for immunofluorescence of Cy dyes, Phycoerythrin (R-PE), phyocyanin (PC), and allophycocyanin (APC).