Product Description: Fluoroshield™ with propidium iodide (PI)
Application Note: Apply 3-4 drops of mounting medium directly on top of the specimen and spread out evenly by tilting back and forth or spread evenly with a 0.2 ml plastic pipette tip making sure the tissue is not touched. Excess medium can be removed by touching the edges of the slide on a paper towel. Let stand at room temperature for about 5 minutes. Apply cover slip, carefully avoiding air bubbles.
Form: Liquid
Concentration:
Background: Fluoroshield mounting medium with PI is an aqueous mounting medium for preserving fluorescence of tissue and cell smears. This unique formula prevents rapid photobleaching of FITC, Texas Red, AMCA, Cy2, Cy3, Cy5, Alexa fluoro 488, Alexa fluoro 594, Green fluorescent protein (GFP), tetramethyly rhodamine, Redox, Phycoerythrin (RP-E), Phyocyanin (PC), and Allophycocyanin (APC). The fluorescence is retained during prolonged storage at 4oC in the dark. This medium does not contain phenylenediamine, which destroys immunofluorescence of Cy dyes, RP-E, PC and APC. This mounting medium is fortified with PI which is a counter-stain for DNA. This product is to be used in in situ hybridization techniques or other methods where fluorescence of DNA staining is required. PI excites at 535nmand emits at 615nm, producing a red fluorescence. RNA is also stained with PI.
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