Application Note: Biotin Glucose Oxidase Conjugated has been tested by ELISA and dot blot and can be utilized in ELISA and Western Blotting experiments where the assay's target of interest is coupled with streptavidin.
Concentration Value: 1.0 mg/mL
ELISA Dilution: 1:8,000 - 1:32,000
Immunohistochemistry Dilution: 1:200 - 1:1,000
Western Blot Dilution: 1:500 - 1:2,500
General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Other Performance Data: 0.01% (w/v) Sodium Azide Added to lot 39296
Physical State: Lyophilized
Purity and Specificity: Biotin Glucose Oxidase Conjugated was prepared from chromatographically purified biotin. Biotin Glucose Oxidase Conjugated assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Glucose Oxidase and anti-Biotin.
Background: Biotin is a small biomolecule important for many cellular processes. Most importantly for biotechnology applications, Biotin is ammenable to conjugation to proteins for use in biochemical assays. Biotin has a very strong affinity for avidin and streptavidin; an attraction that is the strongest and most stable non-covalent interaction known. Glucose oxidase is an enzyme that drives the oxidation of glucose into hydrogen peroxide and D-glucono-δ-lactone. This activity is utilized in biotechnology through colormetric detection assays that are sensitive to the amount of hydrogen peroxide produced. Biotin Glucose Oxidase Conjugated is ideal for investigators involved in Cancer, Cell Signaling, Neuroscience, and Cell Biology research.
Low Endotoxin: No
Other: User Optimized
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