Intended use: For in-vitro Diagnostic Use. The BrightVision one step detection system Goat Anti-Mouse/Rabbit IgG AP, is intended for use in immunohistochemistry for the detection of mouse or rabbit antibodies.
Summary and explanation: The BrightVision detection system Goat Anti- Mouse/Rabbit AP, is a Ready-to-Use system that has been manufactured to give an optimal staining, when using the protocol advised in this IFU. Prior to staining some routine fixed, paraffin-embedding tissue sections should be subjected to pre-treatment (HIER or digestive enzyme). The BrightVision detection system detects Mouse or Rabbit bound to an antigen in tissue sections. This polymer-complex is then visualized with a suitable substrate/chromogen (not provided). Also available in 110 ml, 500 ml and 1000 ml.
Reagents provided: One step detection system Goat anti-Mouse/Rabbit AP (Ready-to-use; 55 ml)
Storage and handling: 2-8°C and in the dark
Principle of method: One step detection system goat anti-mouse/rabbit IgG AP
Procedure: 1. Deparaffinize and rehydrate tissue section (slide/tissue peparing), 2. Wash Aqua dest (Wash; 2x 5 min), 3. If applicable, HIER or digestive enzyme (pre-treatment), 4. Wash buffer (PBS or TBS buffer; 2x 5 min), 5. H2O2 (conc3%) (Tissue preparing; 10 min), 6. Wash buffer (PBS or TBS buffer; 2x 5 min), 7. Primary mouse or rabbit antibody (Antibody; 30 min), 8. Wash buffer (PBS or TBS buffer; 2x 5 min), 9. Detection system, polymer Mouse/Rabbit AP, (Labeled polymer; 30 min), 10. Wash buffer (TBS buffer; 2x 5 min), 11. Substrate (Fast Red / New Fuchsin, see applicable IFU), 12. Wash aqua dest (Wash; 2x 2 min), 13. Counterstain and coverslip with aqueous mounting medium.
References: Shan-Rong Shi et al. Applied Immunohistochemistry & Molecular Morphology, vol 7,201-208,1999
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