Application Note: Rockland Immunochemicals’ Chemiluminescent Western Blot Kit, namely our FemtoMax™ Chemiluminescent Substrate Kit for Western Blotting, combines all of the necessary reagents with a rapid proven protocol and the extremely high signal detection of our luminol substrate. The Chemiluminescent Western Blot Kit design includes straightforward procedures and color-coding to allow for ease of use. This kit contains sufficient substrate for up to 30 mini blots at 7.5 x 8 cm2 and is stable for at least 1 year when stored at +4°C.
Western Blot Dilution: 1:20,000
General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Physical State: n/a
Purity and Specificity: This kit contains: anti-human peroxidase, human IgG control, BSA, tween-20, and Femtomax, along with the protocol and additional supplies. See kit insert for complete details and instructions.
Background: This Chemiluminescent Western Blot Kit allows for the detection of primary human polyclonal or monoclonal antibodies provided by the user. After protein separation and transfer, the membrane is probed with primary antibody. Detection of the membrane bound primary antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with a specialized formulation of luminol, an extremely sensitive, non-radioactive substrate that emits light and allows visualization using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems. Because of the extremely high sensitivity of our FemtoMax™Chemiluminescent Substrate Kit for Western Blotting, primary and secondary antibodies can be used at greater dilutions. If you currently dilute your primary antibody 1:1,000 using an ECL substrate equivalent, then dilute your antibody 1:5,000 using the FemtoMax substrate.
Low Endotoxin: No
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