Application Note: Use Rockland Immuno-chemicals’ Anti-DYKDDDDK (FLAG®) Chemiluminescent Kit for Western Blotting for detection of FLAG® -tagged recombinant proteins by Western blot. This kit is useful for both ''Western blotting'' and ''dot blotting'' methods. After protein separation and transfer, the membrane is probed with Anti-DYKDDDDK (FLAG®). Detection of the membrane bound antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with a specialized formulation of luminol, an extremely sensitive, non-radioactive substrate that emits light and allows visualization using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems.
Concentration Value: n/a
Western Blot Dilution: User Optimized
General Disclaimer Note: This material is subject to proprietary rights and is sold under license from ROCKLAND IMMUNOCHEMICALS Inc. This product is licensed for sale only for 'research-use' only. There is no implied license hereunder for any commercial use. FemtoMax is a trademark of ROCKLAND IMMUNOCHEMICALS Inc. Anti-FLAG® and FLAG® are registered trademarks of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Physical State: n/a
Purity and Specificity: This Chemiluminescent Western Blot Kit allows for the detection of FLAG® -tagged recombinant protein present in cell lysates provided by the user.
Background: The FLAG® epitope is a small but highly immunogenic peptide DYKDDDDK (N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C) which allows fusion proteins to retain their original conformation and function. The hydrophilic character of this sequence increases the likelihood that it will be located on the surface of FLAG® -tagged recombinant proteins for reaction with anti-DYKDDDDK (FLAG®) antibodies. The anti-DYKDDDDK (FLAG®) antibody included in this Western blot kit detects FLAG® -tagged recombinant proteins fused with FLAG® tag either at the amino-terminus or carboxy-terminus of the protein.
Low Endotoxin: No
Other: User Optimized
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