Immunohistochemistry

Immunohistochemistry

Immunohistochemistry (IHC) is an immune-based staining technique enabling antibody detection of specific antigens in tissue samples.

There are three main methods used in IHC: direct, indirect and enzyme „sandwich“ methods.

Direct IHC uses directly labelled (conjugated) primary antibodies while indirect IHC-detection uses unlabeled primary and labelled secondary antibodies. Both primary and secondary antibodies might be conjugated directly either to enzymes most commonly to horseradish peroxidase (HRP) or alkaline phosphatase (AP) or fluorophors. The sandwich method uses specific streptavidin or avidin binding reaction with biotin, which supports signal amplification, and might be preferable when detecting weakly-expressed antigens. Samples using enzymatic detection also need additionally enzyme substrates, which are enzymatically converted into stable chromogen products. These stable products can then be visualized under a light microscope.

We offer a broad range of primary and secondary antibodies, both unconjugated and conjugated to biotin, HRP, AP and various fluorophores. Available in our Webshop are also detection systems (avidin-biotin based, polymersystems), enzyme substrates, mounting media and buffers and solution for your successful immunohistochemical experiments.

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  1. COLEC11 (h): 293T Lysate
    COLEC11 (h): 293T Lysate
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    Sku SACSC-370384
    Manufact. Skusc-370384
    Packaging Unit100 µg/200 µl
  2. Psf2 (h): 293T Lysate
    Psf2 (h): 293T Lysate
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    Sku SACSC-370371
    Manufact. Skusc-370371
    Packaging Unit100 µg/200 µl
  3. DDI1 (h): 293T Lysate
    DDI1 (h): 293T Lysate
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    Sku SACSC-370370
    Manufact. Skusc-370370
    Packaging Unit100 µg/200 µl
  4. Borealin (h): 293T Lysate
    Borealin (h): 293T Lysate
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    Sku SACSC-370361
    Manufact. Skusc-370361
    Packaging Unit100 µg/200 µl
  5. GLTP (h): 293T Lysate
    GLTP (h): 293T Lysate
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    Sku SACSC-370316
    Manufact. Skusc-370316
    Packaging Unit100 µg/200 µl
  6. OTUD7B (h2): 293T Lysate
    OTUD7B (h2): 293T Lysate
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    Sku SACSC-370269
    Manufact. Skusc-370269
    Packaging Unit100 µg/200 µl
  7. p63 (h2): 293T Lysate
    p63 (h2): 293T Lysate
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    Sku SACSC-370267
    Manufact. Skusc-370267
    Packaging Unit100 µg/200 µl
  8. BTG2 (h): 293T Lysate
    BTG2 (h): 293T Lysate
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    Sku SACSC-370262
    Manufact. Skusc-370262
    Packaging Unit100 µg/200 µl
  9. BMP-9 (h2): 293T Lysate
    BMP-9 (h2): 293T Lysate
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    Sku SACSC-370246
    Manufact. Skusc-370246
    Packaging Unit100 µg/200 µl
  10. CREB3L2 (h3): 293T Lysate
    CREB3L2 (h3): 293T Lysate
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    Sku SACSC-370243
    Manufact. Skusc-370243
    Packaging Unit100 µg/200 µl
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