Immunohistochemistry

Immunohistochemistry

Immunohistochemistry (IHC) is an immune-based staining technique enabling antibody detection of specific antigens in tissue samples.

There are three main methods used in IHC: direct, indirect and enzyme „sandwich“ methods.

Direct IHC uses directly labelled (conjugated) primary antibodies while indirect IHC-detection uses unlabeled primary and labelled secondary antibodies. Both primary and secondary antibodies might be conjugated directly either to enzymes most commonly to horseradish peroxidase (HRP) or alkaline phosphatase (AP) or fluorophors. The sandwich method uses specific streptavidin or avidin binding reaction with biotin, which supports signal amplification, and might be preferable when detecting weakly-expressed antigens. Samples using enzymatic detection also need additionally enzyme substrates, which are enzymatically converted into stable chromogen products. These stable products can then be visualized under a light microscope.

We offer a broad range of primary and secondary antibodies, both unconjugated and conjugated to biotin, HRP, AP and various fluorophores. Available in our Webshop are also detection systems (avidin-biotin based, polymersystems), enzyme substrates, mounting media and buffers and solution for your successful immunohistochemical experiments.

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  1. HS1BP3 (m2): 293T Lysate
    HS1BP3 (m2): 293T Lysate
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    Sku SACSC-178765
    Manufact. Skusc-178765
    Packaging Unit100 µg/200 µl
  2. HoxD8 (m): 293T Lysate
    HoxD8 (m): 293T Lysate
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    Sku SACSC-178762
    Manufact. Skusc-178762
    Packaging Unit100 µg/200 µl
  3. HoxD13 (m): 293T Lysate
    HoxD13 (m): 293T Lysate
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    Sku SACSC-178761
    Manufact. Skusc-178761
    Packaging Unit100 µg/200 µl
  4. HAUSP (m): 293T Lysate
    HAUSP (m): 293T Lysate
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    Sku SACSC-178723
    Manufact. Skusc-178723
    Packaging Unit100 µg/200 µl
  5. GSC (m): 293T Lysate
    GSC (m): 293T Lysate
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    Sku SACSC-178707
    Manufact. Skusc-178707
    Packaging Unit100 µg/200 µl
  6. GRK 3 (m): 293 Lysate
    GRK 3 (m): 293 Lysate
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    Sku SACSC-178704
    Manufact. Skusc-178704
    Packaging Unit100 µg/200 µl
  7. Glut6 (m): 293 Lysate
    Glut6 (m): 293 Lysate
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    Sku SACSC-178677
    Manufact. Skusc-178677
    Packaging Unit100 µg/200 µl
  8. GDF-11 (m): 293 Lysate
    GDF-11 (m): 293 Lysate
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    Sku SACSC-178664
    Manufact. Skusc-178664
    Packaging Unit100 µg/200 µl
  9. gasdermin (m): 293 Lysate
    gasdermin (m): 293 Lysate
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    Sku SACSC-178656
    Manufact. Skusc-178656
    Packaging Unit100 µg/200 µl
  10. GALP (m2): 293T Lysate
    GALP (m2): 293T Lysate
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    Sku SACSC-178651
    Manufact. Skusc-178651
    Packaging Unit100 µg/200 µl
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