Immunohistochemistry

Immunohistochemistry

Immunohistochemistry (IHC) is an immune-based staining technique enabling antibody detection of specific antigens in tissue samples.

There are three main methods used in IHC: direct, indirect and enzyme „sandwich“ methods.

Direct IHC uses directly labelled (conjugated) primary antibodies while indirect IHC-detection uses unlabeled primary and labelled secondary antibodies. Both primary and secondary antibodies might be conjugated directly either to enzymes most commonly to horseradish peroxidase (HRP) or alkaline phosphatase (AP) or fluorophors. The sandwich method uses specific streptavidin or avidin binding reaction with biotin, which supports signal amplification, and might be preferable when detecting weakly-expressed antigens. Samples using enzymatic detection also need additionally enzyme substrates, which are enzymatically converted into stable chromogen products. These stable products can then be visualized under a light microscope.

We offer a broad range of primary and secondary antibodies, both unconjugated and conjugated to biotin, HRP, AP and various fluorophores. Available in our Webshop are also detection systems (avidin-biotin based, polymersystems), enzyme substrates, mounting media and buffers and solution for your successful immunohistochemical experiments.

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  1. Latexin (h2): 293T Lysate
    Latexin (h2): 293T Lysate
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    Sku SACSC-177465
    Manufact. Skusc-177465
    Packaging Unit100 µg/200 µl
  2. Latexin (h): 293T Lysate
    Latexin (h): 293T Lysate
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    Sku SACSC-177464
    Manufact. Skusc-177464
    Packaging Unit100 µg/200 µl
  3. Laminin β-3 (h2): 293T Lysate
    Laminin β-3 (h2): 293T Lysate
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    Sku SACSC-177459
    Manufact. Skusc-177459
    Packaging Unit100 µg/200 µl
  4. Laminin β-3 (h): 293T Lysate
    Laminin β-3 (h): 293T Lysate
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    Sku SACSC-177458
    Manufact. Skusc-177458
    Packaging Unit100 µg/200 µl
  5. Lamin A (h2): 293T Lysate
    Lamin A (h2): 293T Lysate
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    Sku SACSC-177453
    Manufact. Skusc-177453
    Packaging Unit100 µg/200 µl
  6. Lamin A (h): 293T Lysate
    Lamin A (h): 293T Lysate
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    Sku SACSC-177452
    Manufact. Skusc-177452
    Packaging Unit100 µg/200 µl
  7. Lad (h): 293T Lysate
    Lad (h): 293T Lysate
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    Sku SACSC-177448
    Manufact. Skusc-177448
    Packaging Unit100 µg/200 µl
  8. Ku70 (h5): 293T Lysate
    Ku70 (h5): 293T Lysate
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    Sku SACSC-177443
    Manufact. Skusc-177443
    Packaging Unit100 µg/200 µl
  9. KLC4 (h4): 293T Lysate
    KLC4 (h4): 293T Lysate
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    Sku SACSC-177434
    Manufact. Skusc-177434
    Packaging Unit100 µg/200 µl
  10. KLC4 (h5): 293T Lysate
    KLC4 (h5): 293T Lysate
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    Sku SACSC-177433
    Manufact. Skusc-177433
    Packaging Unit100 µg/200 µl
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