Immunohistochemistry

Immunohistochemistry

Immunohistochemistry (IHC) is an immune-based staining technique enabling antibody detection of specific antigens in tissue samples.

There are three main methods used in IHC: direct, indirect and enzyme „sandwich“ methods.

Direct IHC uses directly labelled (conjugated) primary antibodies while indirect IHC-detection uses unlabeled primary and labelled secondary antibodies. Both primary and secondary antibodies might be conjugated directly either to enzymes most commonly to horseradish peroxidase (HRP) or alkaline phosphatase (AP) or fluorophors. The sandwich method uses specific streptavidin or avidin binding reaction with biotin, which supports signal amplification, and might be preferable when detecting weakly-expressed antigens. Samples using enzymatic detection also need additionally enzyme substrates, which are enzymatically converted into stable chromogen products. These stable products can then be visualized under a light microscope.

We offer a broad range of primary and secondary antibodies, both unconjugated and conjugated to biotin, HRP, AP and various fluorophores. Available in our Webshop are also detection systems (avidin-biotin based, polymersystems), enzyme substrates, mounting media and buffers and solution for your successful immunohistochemical experiments.

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  1. COL6A2 (h2): 293T Lysate
    COL6A2 (h2): 293T Lysate
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    Sku SACSC-177076
    Manufact. Skusc-177076
    Packaging Unit100 µg/200 µl
  2. CNOT10 (h4): 293T Lysate
    CNOT10 (h4): 293T Lysate
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    Sku SACSC-177070
    Manufact. Skusc-177070
    Packaging Unit100 µg/200 µl
  3. CHES1 (h2): 293T Lysate
    CHES1 (h2): 293T Lysate
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    Sku SACSC-177057
    Manufact. Skusc-177057
    Packaging Unit100 µg/200 µl
  4. CHDH (h): 293T Lysate
    CHDH (h): 293T Lysate
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    Sku SACSC-177054
    Manufact. Skusc-177054
    Packaging Unit100 µg/200 µl
  5. Centaurin γ3 (h): 293T Lysate
    Centaurin γ3 (h): 293T Lysate
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    Sku SACSC-177048
    Manufact. Skusc-177048
    Packaging Unit100 µg/200 µl
  6. Cdc25C (h3): 293T Lysate
    Cdc25C (h3): 293T Lysate
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    Sku SACSC-177045
    Manufact. Skusc-177045
    Packaging Unit100 µg/200 µl
  7. CCDC8 (h): 293T Lysate
    CCDC8 (h): 293T Lysate
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    Sku SACSC-177038
    Manufact. Skusc-177038
    Packaging Unit100 µg/200 µl
  8. CCDC57 (h2): 293T Lysate
    CCDC57 (h2): 293T Lysate
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    Sku SACSC-177034
    Manufact. Skusc-177034
    Packaging Unit100 µg/200 µl
  9. Cbp (h2): 293T Lysate
    Cbp (h2): 293T Lysate
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    Sku SACSC-177033
    Manufact. Skusc-177033
    Packaging Unit100 µg/200 µl
  10. Cbp (h): 293T Lysate
    Cbp (h): 293T Lysate
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    Sku SACSC-177032
    Manufact. Skusc-177032
    Packaging Unit100 µg/200 µl
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