Immunohistochemistry

Immunohistochemistry

Immunohistochemistry (IHC) is an immune-based staining technique enabling antibody detection of specific antigens in tissue samples.

There are three main methods used in IHC: direct, indirect and enzyme „sandwich“ methods.

Direct IHC uses directly labelled (conjugated) primary antibodies while indirect IHC-detection uses unlabeled primary and labelled secondary antibodies. Both primary and secondary antibodies might be conjugated directly either to enzymes most commonly to horseradish peroxidase (HRP) or alkaline phosphatase (AP) or fluorophors. The sandwich method uses specific streptavidin or avidin binding reaction with biotin, which supports signal amplification, and might be preferable when detecting weakly-expressed antigens. Samples using enzymatic detection also need additionally enzyme substrates, which are enzymatically converted into stable chromogen products. These stable products can then be visualized under a light microscope.

We offer a broad range of primary and secondary antibodies, both unconjugated and conjugated to biotin, HRP, AP and various fluorophores. Available in our Webshop are also detection systems (avidin-biotin based, polymersystems), enzyme substrates, mounting media and buffers and solution for your successful immunohistochemical experiments.

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  1. IER2 (h4): 293T Lysate
    IER2 (h4): 293T Lysate
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    Sku SACSC-172808
    Manufact. Skusc-172808
    Packaging Unit100 µg/200 µl
  2. ABCG2 (h3): 293T Lysate
    ABCG2 (h3): 293T Lysate
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    Sku SACSC-172804
    Manufact. Skusc-172804
    Packaging Unit100 µg/200 µl
  3. HOOK2 (h4): 293T Lysate
    HOOK2 (h4): 293T Lysate
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    Sku SACSC-172802
    Manufact. Skusc-172802
    Packaging Unit100 µg/200 µl
  4. MPP2 (h3): 293T Lysate
    MPP2 (h3): 293T Lysate
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    Sku SACSC-172797
    Manufact. Skusc-172797
    Packaging Unit100 µg/200 µl
  5. NMT2 (h2): 293T Lysate
    NMT2 (h2): 293T Lysate
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    Sku SACSC-172790
    Manufact. Skusc-172790
    Packaging Unit100 µg/200 µl
  6. Presenilin 2 (h2): 293T Lysate
    Presenilin 2 (h2): 293T Lysate
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    Sku SACSC-172789
    Manufact. Skusc-172789
    Packaging Unit100 µg/200 µl
  7. casein kinase Iγ2 (h2): 293T Lysate
    casein kinase Iγ2 (h2): 293T Lysate
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    Sku SACSC-172788
    Manufact. Skusc-172788
    Packaging Unit100 µg/200 µl
  8. TRP2 (h3): 293T Lysate
    TRP2 (h3): 293T Lysate
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    Sku SACSC-172779
    Manufact. Skusc-172779
    Packaging Unit100 µg/200 µl
  9. PP2Cα (h2): 293T Lysate
    PP2Cα (h2): 293T Lysate
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    Sku SACSC-172768
    Manufact. Skusc-172768
    Packaging Unit100 µg/200 µl
  10. TRAF2 (h6): 293T Lysate
    TRAF2 (h6): 293T Lysate
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    Sku SACSC-172765
    Manufact. Skusc-172765
    Packaging Unit100 µg/200 µl
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