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Application: Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.
Background: ULK1 (unc-51 like autophagy activating kinase 1) is a serine/threonine protein kinase that plays a critical role during the initial stages of autophagy in response to nutrient starvation. The conserved C-terminal domain of ULK1 controls the function and the localization of the protein. Knockdown of ULK1 inhibits the autophagic response and prevents rapamycin-induced autophagy, consistent with a role downstream of mTOR (mammalian target of rapamycin). ULK1 forms a complex with FIP200 (focal adhesion kinase family interacting protein of 200 kDa) and ATG13 (autophagy-related protein 13) and this complex is essential for starvation-induced autophagy. Both FIP200 and ATG13 are critical for correct localization of ULK1 to the pre-autophagosome and stability of the ULK1 protein. ULK1 is phosphorylated upon activation of the mTOR pathway in a starvation-regulated manner. Alterations in ULK signaling pathways may be involved in the formation of autophagy-regulated Lewi bodies, which have been associated with Parkinson's disease. A deeper understanding of the roles of this protein and development of therapeutical strategies around it may benefit patients suffering from ULK1 related disorders.
Contraindications: The final concentration of DMSO in the assay should not exceed 1%.
Description: The Chemi-Verse™ ULK1 Kinase Assay Kit is designed to measure ULK1 (Unc-51-like autophagy-activating kinase 1) serine/threonine kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified ULK1, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Uniprot: O75385
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
References: Ganley I.G., et al., 2009 J. Biol. Chem. 284 (18): 12297-12305.
Chan E.Y., et al., 2009 Mol. Cell. Biol. 29 (1): 157-171.
Miki Y., et al., 2016 Brain Pathol. 26: 359-70.
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