Assay Time: 30min
Test Principle: Elabscience? Calcein AM Solution (100 uM) can be used to distinguish living cells in mammals with esterase activity. Calcein AM is the addition of acetyl methoxy methyl ester (AM) group to traditional Calcein, which increases hydrophobicity and can easily penetrate the living cell membrane and enter the cell. Calcein AM itself has no fluorescence. After entering the cell, it is hydrolyzed by endogenous esterase in the cell to produce Calcein, a polar molecule with strong negative charge and cannot be retained in the cell through the cell membrane, while Calcein can emit strong green fluorescence ( Ex/Em = 494nm/517nm ). Due to the lack of esterase, dead cells cannot or rarely produce Calcein, so only living cells are stained with strong green fluorescence, and dead cells cannot be stained or stained very weakly. Compared with other live cell staining probes, Calcein AM is less toxic and does not affect cell differentiation and proliferation.
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