Abbreviation: 5-HETE
Target Synonym: 5-HETE;5(S)-HETE;5S-HETE;
Target Species: Universal
Usage: This ELISA kit applies to the in vitro quantitative determination of 5-HETE concentrations in Serum, plasma and other biological fluids.
Tested Sample Types: Serum, plasma and other biological fluids
Assay Time: 2.5h
Sensitivity: 0.09 ng/mL
Detection Range: 0.16-10 ng/mL
Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal 5-HETE. During the reaction, Universal 5-HETE in the sample or standard competes with a fixed amount of Universal 5-HETE on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal 5-HETE. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal 5-HETE in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Cross reactivity: This kit recognizes Universal 5-HETE in samples.No significant cross-reactivity or interference between Universal 5-HETE and analogues was observed
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