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Applications: Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Assay Conditions: Inhibition of ACLY enzyme by BMS303141, measured using the ACLY Kinase Kit, BPS
Bioscience #79904. Data shown is lot-specific. For lot-specific information, please contact
BPS Bioscience, Inc. at support@bpsbioscience.com
Description: ATP citrate lyase (ACLY) is an important enzyme in fatty acid synthesis and cancer metabolism. The ACLY Assay Kit is designed to measure ACLY activity for screening and profiling applications using ADP-Glo® Kinase Assay as a detection reagent.
Storage Stability: Up to 6 months when stored as recommended.
Supplied As: The ACLY Assay Kit comes in a convenient 96-well format, with enough purified recombinant ACLY enzyme, substrate, ATP, and kinase assay buffer for 100 enzyme reactions.
Warnings: Avoid multiple freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Zaidi, N., J. Swinnen, and K. Smans. 2012. ATP-Citrate Lyase: A Key Player in Cancer Metabolism. Cancer Research 72 (15):3709-14.
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