Abbreviation: Cortisol
Target Species: Bovine and Sheep
Usage: This ELISA kit applies to the in vitro quantitative determination of Bovine and Sheep Cortisol concentrations in serum, plasma.
Tested Sample Types: serum, plasma
Assay Time: 2.5h
Sensitivity: 0.96 ng/mL
Detection Range: 1.56-100 ng/mL
Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Bovine and Sheep Cortisol. During the reaction, Bovine and Sheep Cortisol in the sample or standard competes with a fixed amount of Bovine and Sheep Cortisol on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Bovine and Sheep Cortisol. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Bovine and Sheep Cortisol in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Cross reactivity: This kit recognizes Bovine and Sheep Cortisol in samples.No significant cross-reactivity or interference between Bovine and Sheep Cortisol and analogues was observed
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